Hotspot

rrn5-trnR-ACG

IRb · 106268-106531 · 264 bp

Back to Eupatorium

Pi 0.00515

MarkerSeek score 57.50

Primer available yes

Label rrn5-trnR-ACG

Feature Metadata

label_name: rrn5-trnR-ACG
feature_type: igs
region: IRb
length_bp: 264
variable_sites: 2
pi: 0.00515
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR-ACG_p1	GGTGACGATACTGTAGGGGA	CCACGTGCTCTAATCCTCTG	55.0	55.0	57.9	57.8	343-348 bp	1.0	79.4
rrn5-trnR-ACG_p2	AATCCATCCCGAACTTGGTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	378-383 bp	1.0	78.7
rrn5-trnR-ACG_p3	ACCAATCCATCCCGAACTTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	381-386 bp	1.0	78.7
rrn5-trnR-ACG_p4	CAATCCATCCCGAACTTGGT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	379-384 bp	1.0	78.7
rrn5-trnR-ACG_p5	CACCAATCCATCCCGAACTT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	382-387 bp	1.0	78.7