Hotspot

ycf3-trnS-GGA

LSC · 43774-44665 · 892 bp

Back to Eupatorium

Pi 0.00983

MarkerSeek score 53.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 13
pi: 0.00983
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	ATGCTACGCTTTCAACCACT	45.0	45.0	56.4	57.8	1022 bp	1.0	67.6
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	ATGCTACGCTTTCAACCACT	45.0	45.0	56.4	57.8	1023 bp	1.0	67.6
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1065 bp	1.0	67.3
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1066 bp	1.0	67.3
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	CCAATGCTACGCTTTCAACC	45.0	50.0	56.4	57.7	1025 bp	1.0	67.0