Hotspot

atpI-atpH

LSC · 24925-26074 · 1150 bp

Back to Eupatorium

Pi 0.00348

MarkerSeek score 61.00

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1150
variable_sites: 6
pi: 0.00348
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	CCCGTCATGTTCCTTGGATT	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.9	58.0	1284-1287 bp	1.0	79.0
atpI-atpH_p2	CCCGTCATGTTCCTTGGATT	TACCTTGACCAACTCCAGGT	50.0	50.0	57.9	57.9	1340-1343 bp	1.0	78.4
atpI-atpH_p3	CCCGTCATGTTCCTTGGATT	AGCCAATCCAGCAGCAATAA	50.0	45.0	57.9	57.9	1300-1303 bp	1.0	78.2
atpI-atpH_p4	CCCGTCATGTTCCTTGGATT	AATAACGGAAGCGGCAGAAA	50.0	45.0	57.9	58.2	1285-1288 bp	1.0	77.9
atpI-atpH_p5	CCCGTCATGTTCCTTGGATT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.9	58.2	1344-1347 bp	1.0	77.8