Hotspot

trnY-GUA-trnE-UUC

LSC · 11568-11748 · 181 bp

Back to Eupatorium

Pi 0.00737

MarkerSeek score 37.70

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 2
pi: 0.00737
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CGAATTTACAGTCCGTCCCC	AAAACGAGGGTTCAAGTCCC	55.0	50.0	58.4	58.0	255 bp	1.0	77.6
trnY-GUA-trnE-UUC_p2	ACGAATTTACAGTCCGTCCC	AAAACGAGGGTTCAAGTCCC	50.0	50.0	57.6	58.0	256 bp	1.0	77.4
trnY-GUA-trnE-UUC_p3	CGAATTTACAGTCCGTCCCC	CCCCTATGGTCCAGTGGTTA	55.0	55.0	58.4	58.1	296 bp	1.0	76.8
trnY-GUA-trnE-UUC_p4	CGAATTTACAGTCCGTCCCC	CGGAGAAAACGAGGGTTCAA	55.0	50.0	58.4	58.1	260 bp	1.0	76.8
trnY-GUA-trnE-UUC_p5	ATTGACAAATTGTTGGGCCG	AAAACGAGGGTTCAAGTCCC	45.0	50.0	57.5	58.0	309 bp	1.0	76.7