Hotspot

ndhF-rpl32

SSC · 114584-115617 · 1034 bp

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Pi 0.0301

MarkerSeek score 73.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1034
variable_sites: 109
pi: 0.0301
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGATGATCCCGGAATATTGA	TGATATAGAAGTGCGCTTTT	45.0	35.0	54.4	52.7	1010-1136 bp	1.0	52.0
ndhF-rpl32_p2	GGATGATCCCGGAATATTGA	TTGATATAGAAGTGCGCTTT	45.0	35.0	54.4	52.7	1011-1137 bp	1.0	52.0
ndhF-rpl32_p3	GGATGATCCCGGAATATTGA	TTTGATATAGAAGTGCGCTT	45.0	35.0	54.4	52.7	1012-1138 bp	1.0	52.0
ndhF-rpl32_p4	GGATGATCCCGGAATATTGA	TTTTGATATAGAAGTGCGCT	45.0	35.0	54.4	52.7	1013-1139 bp	1.0	52.0
ndhF-rpl32_p5	GGATGATCCCGGAATATTGA	TGATATAGAAGTGCGCTTTTT	45.0	33.3	54.4	53.6	1010-1136 bp	1.0	52.0