Hotspot

trnG-GCC-trnfM-CAU

LSC · 37864-38030 · 167 bp

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Pi 0.0454

MarkerSeek score 72.70

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 17
pi: 0.0454
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	236-257 bp	1.0	87.2
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	234-255 bp	1.0	86.2
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.8	57.8	249-270 bp	1.0	85.2
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	GCGAGGCTCATAACCTTGAG	50.0	55.0	57.8	58.4	250-271 bp	1.0	83.6
trnG-GCC-trnfM-CAU_p5	TGCGGGTATAGTCGAATGGT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.6	58.0	264-285 bp	1.0	82.9