Hotspot

psbC-trnS-UGA

LSC · 36133-36384 · 252 bp

Back to Euonymus

Pi 0.0278

MarkerSeek score 68.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 252
variable_sites: 23
pi: 0.0278
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	TTGATCGCGATTTTGAGCCT	AGCTCTGGTCTTGAAAACCG	45.0	50.0	58.3	58.1	360-391 bp	1.0	86.2
psbC-trnS-UGA_p2	TTGATCGCGATTTTGAGCCT	GATGGCCGAGTGGTTGATAG	45.0	55.0	58.3	58.1	378-409 bp	1.0	85.8
psbC-trnS-UGA_p3	GCTGCGGGATTTGAAAAAGG	AGCTCTGGTCTTGAAAACCG	50.0	50.0	58.3	58.1	382-413 bp	1.0	85.7
psbC-trnS-UGA_p4	GCTGCGGGATTTGAAAAAGG	GATGGCCGAGTGGTTGATAG	50.0	55.0	58.3	58.1	400-431 bp	1.0	85.2
psbC-trnS-UGA_p5	TTGATCGCGATTTTGAGCCT	CGAGTGGTTGATAGCTCTGG	45.0	55.0	58.3	57.8	372-403 bp	1.0	85.2