Hotspot

psbM-trnD-GUC

LSC · 30400-30867 · 468 bp

Back to Euonymus

Pi 0.0232

MarkerSeek score 69.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 47
pi: 0.0232
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGCGAGAATATTGACTTCCA	GTTCGAGCCCCGTCAGTC	40.0	66.7	54.5	60.1	485-541 bp	1.0	51.6
psbM-trnD-GUC_p2	ATGCGAGAATATTGACTTCCA	GTTCGAGCCCCGTCAGTC	38.1	66.7	55.1	60.1	486-542 bp	1.0	51.6
psbM-trnD-GUC_p3	TGCGAGAATATTGACTTCCAT	GTTCGAGCCCCGTCAGTC	38.1	66.7	55.1	60.1	485-541 bp	1.0	51.6
psbM-trnD-GUC_p4	AATGCGAGAATATTGACTTCCA	GTTCGAGCCCCGTCAGTC	36.4	66.7	55.9	60.1	487-543 bp	1.0	51.5
psbM-trnD-GUC_p5	TGCGAGAATATTGACTTCCA	GAGCACCGCCCTGTCAAG	40.0	66.7	54.5	60.7	516-572 bp	1.0	51.1