Hotspot

petN-psbM

LSC · 29396-30279 · 884 bp

Back to Euonymus

Pi 0.0282

MarkerSeek score 71.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 884
variable_sites: 86
pi: 0.0282
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	AGTCAGGGCGATAAAGTTGA	50.0	45.0	58.2	56.6	933-1041 bp	1.0	78.3
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	AGTCAGGGCGATAAAGTTGAA	50.0	42.9	58.2	57.3	933-1041 bp	1.0	76.3
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AGTCAAAGTCAGGGCGATAA	50.0	45.0	58.2	56.6	939-1032 bp	0.933333	75.5
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	AGTCAGGGCGATAAAGTTGA	50.0	45.0	57.6	56.6	872-980 bp	0.933333	74.3
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	AGTCAAAGTCAGGGCGATAA	50.0	45.0	57.6	56.6	878-971 bp	0.866667	71.5