Hotspot

trnG-UCC-trnR-UCU

LSC · 9659-10050 · 392 bp

Back to Euonymus

Pi 0.0288

MarkerSeek score 68.60

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 51
pi: 0.0288
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TGTCCTATCCATTAGACAATG	50.0	38.1	58.0	52.4	462-581 bp	1.0	52.0
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	TGTCCTATCCATTAGACAATG	55.0	38.1	58.1	52.4	465-584 bp	1.0	52.0
trnG-UCC-trnR-UCU_p3	CCCTAGCCTTCCAAGCTAAC	TGTCCTATCCATTAGACAATG	55.0	38.1	57.7	52.4	466-585 bp	1.0	52.0
trnG-UCC-trnR-UCU_p4	CTAACGATGCGGGTTCGATT	TGTCCTATCCATTAGACAATG	50.0	38.1	58.4	52.4	451-570 bp	1.0	52.0
trnG-UCC-trnR-UCU_p5	TAACGATGCGGGTTCGATTC	TGTCCTATCCATTAGACAATG	50.0	38.1	58.4	52.4	450-569 bp	1.0	52.0