Hotspot

rps16-trnQ-UUG

LSC · 5349-6357 · 1009 bp

Back to Euonymus

Pi 0.0205

MarkerSeek score 69.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1009
variable_sites: 84
pi: 0.0205
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TAGTTCGTTGCTTTCGACCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.5	58.1	897-1247 bp	1.0	85.7
rps16-trnQ-UUG_p2	ATAGTTCGTTGCTTTCGACCA	GAGGTTCGAATCCTTCCGTC	42.9	55.0	58.0	58.1	898-1248 bp	1.0	81.5
rps16-trnQ-UUG_p3	AGTTCGTTGCTTTCGACCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	59.1	58.1	896-1246 bp	1.0	81.3
rps16-trnQ-UUG_p4	TTCGTTGCTTTCGACCACA	GAGGTTCGAATCCTTCCGTC	47.4	55.0	57.9	58.1	894-1244 bp	1.0	81.3
rps16-trnQ-UUG_p5	TAGTTCGTTGCTTTCGACCA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	57.5	57.3	901-1251 bp	1.0	80.9