Hotspot

rpoC2

LSC · 15714-19805 · 4092 bp

Back to Eulophia

Pi 0.0122

MarkerSeek score 48.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4092
variable_sites: 49
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CCTTGCGTTATGTCACCAGA	TGTCTATTGAAATACGGATCAC	50.0	36.4	57.9	53.8	206 bp	1.0	44.7
rpoC2_p2	AGACCTTGCGTTATGTCACC	TGTCTATTGAAATACGGATCAC	50.0	36.4	57.9	53.8	209 bp	1.0	44.6
rpoC2_p3	ACCTTGCGTTATGTCACCAG	TGTCTATTGAAATACGGATCAC	50.0	36.4	58.2	53.8	207 bp	1.0	44.6
rpoC2_p4	GACCTTGCGTTATGTCACCA	TGTCTATTGAAATACGGATCAC	50.0	36.4	58.2	53.8	208 bp	1.0	44.6
rpoC2_p5	ACTTTTGGAAGACCTTGCGT	TGTCTATTGAAATACGGATCAC	45.0	36.4	57.9	53.8	218 bp	1.0	44.4