Hotspot

ccsA-ndhD

SSC · 112275-112544 · 270 bp

Back to Eulophia

Pi 0.0502

MarkerSeek score 73.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 270
variable_sites: 13
pi: 0.0502
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCGGCATTTGTAGCTTCT	TCGGTTGACAAGGTACAAGT	40.0	45.0	55.4	56.7	428-431 bp	1.0	53.6
ccsA-ndhD_p2	AATTCGGCATTTGTAGCTTCT	TCGGTTGACAAGGTACAAGT	38.1	45.0	56.2	56.7	429-432 bp	1.0	51.9
ccsA-ndhD_p3	ATTCGGCATTTGTAGCTTCT	TCGGTTGACAAGGTACAAGTT	40.0	42.9	55.4	57.5	428-431 bp	1.0	51.5
ccsA-ndhD_p4	AATTCGGCATTTGTAGCTTCT	TCGGTTGACAAGGTACAAGTT	38.1	42.9	56.2	57.5	429-432 bp	1.0	49.8
ccsA-ndhD_p5	AAATTCGGCATTTGTAGCTTCT	TCGGTTGACAAGGTACAAGT	36.4	45.0	56.9	56.7	430-433 bp	1.0	49.7