Hotspot

trnN-GUU-rpl32

IRb · 109715-110233 · 519 bp

Back to Eulophia

Pi 0.0168

MarkerSeek score 62.20

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: IRb
length_bp: 519
variable_sites: 8
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	AGTCAGTTAACAGCCAACCG	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	58.1	57.3	611-654 bp	1.0	54.1
trnN-GUU-rpl32_p2	ACGACCAGTCAGTTAACAGC	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	57.8	57.3	617-660 bp	1.0	53.9
trnN-GUU-rpl32_p3	AGGATTCGAACCTACGACCA	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	58.2	57.3	630-673 bp	1.0	53.8
trnN-GUU-rpl32_p4	AGTCAGTTAACAGCCAACCG	AGAAAAAGCTTTTACTGCCTCT	50.0	36.4	58.1	56.2	618-661 bp	1.0	53.5
trnN-GUU-rpl32_p5	GGATTCGAACCTACGACCAG	GCTTTTACTGCCTCTAAAGATCC	55.0	43.5	57.8	57.3	629-672 bp	1.0	53.4