Hotspot

psbK-psbI

LSC · 7378-7812 · 435 bp

Back to Eulophia

Pi 0.0144

MarkerSeek score 67.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 435
variable_sites: 6
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	537-555 bp	1.0	82.5
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	538-556 bp	1.0	82.5
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTACGCCCCGGATCATTAGA	50.0	50.0	57.9	58.3	539-557 bp	1.0	80.7
psbK-psbI_p4	TTATGCCGGTTATCCCCGTA	ACGCCCCGGATCATTAGATA	50.0	50.0	58.3	58.1	578-596 bp	1.0	80.5
psbK-psbI_p5	TTATGCCGGTTATCCCCGTA	TACGCCCCGGATCATTAGAT	50.0	50.0	58.3	58.1	579-597 bp	1.0	80.4