Hotspot

ndhG-ndhI

SSC · 113321-113510 · 190 bp

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Pi 0.0273

MarkerSeek score 71.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 190
variable_sites: 5
pi: 0.0273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCCAGCGAAAAGGCAGAATA	GAGTCGGTTACCAATCTCCA	50.0	50.0	58.2	56.7	427-2926 bp	1.0	67.8
ndhG-ndhI_p2	CCCAGCGAAAAGGCAGAATA	TGAGTCGGTTACCAATCTCC	50.0	50.0	58.2	56.7	428-2926 bp	1.0	67.8
ndhG-ndhI_p3	CCCAGCGAAAAGGCAGAATA	TGAGTCGGTTACCAATCTCCA	50.0	47.6	58.2	58.5	428-2927 bp	1.0	66.8
ndhG-ndhI_p4	CCCAGCGAAAAGGCAGAATA	TTGAGTCGGTTACCAATCTCC	50.0	47.6	58.2	57.4	429-2927 bp	1.0	65.7
ndhG-ndhI_p5	CCCAGCGAAAAGGCAGAATA	GAGTCGGTTACCAATCTCCAT	50.0	47.6	58.2	57.2	427-2926 bp	1.0	64.0