Hotspot

ndhF-rpl32

SSC · 107088-107935 · 848 bp

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Pi 0.0178
MarkerSeek score 67.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
848
variable_sites
15
pi
0.0178
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGGAGAAGAGGA TCCAAAGATTTTTACGAATACGCT 55.0 33.3 58.1 57.3 961-2928 bp 1.0 42.6
ndhF-rpl32_p2 CTGGAAGTGGGAGAAGAGGA TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 933-2924 bp 1.0 42.3
ndhF-rpl32_p3 CTGGAAGTGGGAGAAGAGGA TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 934-2924 bp 1.0 42.3
ndhF-rpl32_p4 CTGGAAGTGGGAGAAGAGGA TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 935-2924 bp 1.0 42.3
ndhF-rpl32_p5 CTGGAAGTGGGAGAAGAGGA TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 936-2924 bp 1.0 42.3