Hotspot

petL-petG

LSC · 65192-65358 · 167 bp

Back to Eulalia

Pi 0.018

MarkerSeek score 58.90

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 3
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	CGGTTTTCTACTGGCTGCTT	ACGCAGTCACGAATAATCCC	50.0	50.0	58.5	58.1	316-2925 bp	1.0	75.8
petL-petG_p2	TTCGGTTTTCTACTGGCTGC	ACGCAGTCACGAATAATCCC	50.0	50.0	58.5	58.1	318-2925 bp	1.0	75.8
petL-petG_p3	CGGTTTTCTACTGGCTGCTT	CAGTCACGAATAATCCCGCT	50.0	50.0	58.5	57.8	313-2924 bp	1.0	74.5
petL-petG_p4	TTCGGTTTTCTACTGGCTGC	CAGTCACGAATAATCCCGCT	50.0	50.0	58.5	57.8	315-2924 bp	1.0	74.5
petL-petG_p5	CGGTTTTCTACTGGCTGCTT	CGCCTGTATTGCAAATACGC	50.0	50.0	58.5	58.3	332-2925 bp	1.0	74.3