Hotspot

trnV-UAC-trnM-CAU

LSC · 53411-53634 · 224 bp

Back to Eulalia

Pi 0.0391

MarkerSeek score 71.50

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 224
variable_sites: 7
pi: 0.0391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	AACGAGTTGCTCTACCGAAC	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.9	58.4	302-2925 bp	1.0	78.8
trnV-UAC-trnM-CAU_p2	AACGAGTTGCTCTACCGAAC	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.9	57.3	301-2927 bp	1.0	76.8
trnV-UAC-trnM-CAU_p3	CGAGTTGCTCTACCGAACTG	CCCGCCGTATGAAAGCAATA	55.0	50.0	58.4	58.4	300-2923 bp	1.0	76.2
trnV-UAC-trnM-CAU_p4	AACGAGTTGCTCTACCGAAC	TTTGAACCAATGACTCCCGC	50.0	50.0	57.9	58.8	317-2927 bp	1.0	76.0
trnV-UAC-trnM-CAU_p5	AACGAGTTGCTCTACCGAAC	AGCGAAGTAAGTCCAATGCT	50.0	45.0	57.9	57.2	374-2926 bp	1.0	75.9