Hotspot

ndhC-trnV-UAC

LSC · 51822-52732 · 911 bp

Back to Eulalia

Pi 0.0144

MarkerSeek score 58.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 13
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCAAAATAGGAATAAGGCTTGC	GGTTCGAATCCGTATAGCCC	39.1	55.0	57.4	57.9	1004-2927 bp	1.0	51.4
ndhC-trnV-UAC_p2	ACCAAAATAGGAATAAGGCTTGC	AGCCCAATGCAGAAAAATGC	39.1	45.0	57.4	57.9	1097-2927 bp	1.0	51.1
ndhC-trnV-UAC_p3	ACCAAAATAGGAATAAGGCTTGC	CGGTTCGAATCCGTATAGCC	39.1	55.0	57.4	58.3	1005-2927 bp	1.0	49.5
ndhC-trnV-UAC_p4	CCAAAATAGGAATAAGGCTTGCT	GGTTCGAATCCGTATAGCCC	39.1	55.0	57.1	57.9	1003-2926 bp	1.0	49.3
ndhC-trnV-UAC_p5	TCATATTCGTGAAGCAGAAACA	GGTTCGAATCCGTATAGCCC	36.4	55.0	56.1	57.9	956-2926 bp	1.0	49.1