Hotspot

ndhJ-ndhK

LSC · 50624-50721 · 98 bp

Back to Eulalia

Pi 0.051

MarkerSeek score 81.40

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 98
variable_sites: 5
pi: 0.051
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	ATGATCGAAGCCCAAAGACC	TCACACTGGAACTTACGAACA	50.0	42.9	58.0	57.2	288-2927 bp	1.0	65.5
ndhJ-ndhK_p2	CGATGATCGAAGCCCAAAGA	TCACACTGGAACTTACGAACA	50.0	42.9	58.1	57.2	290-2926 bp	1.0	65.3
ndhJ-ndhK_p3	TCGATGATCGAAGCCCAAAG	TCACACTGGAACTTACGAACA	50.0	42.9	58.1	57.2	291-2927 bp	1.0	65.3
ndhJ-ndhK_p4	ATGATCGAAGCCCAAAGACC	ACTCACACTGGAACTTACGA	50.0	45.0	58.0	56.1	290-2926 bp	1.0	65.1
ndhJ-ndhK_p5	CGATGATCGAAGCCCAAAGA	ACTCACACTGGAACTTACGA	50.0	45.0	58.1	56.1	292-2925 bp	1.0	65.0