Hotspot

petN-trnC-GCA

LSC · 19134-20010 · 877 bp

Back to Eulalia

Pi 0.0222

MarkerSeek score 64.60

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 877
variable_sites: 19
pi: 0.0222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	CTGCAAATCCTTTACCCCCA	50.0	50.0	58.2	57.8	945-2927 bp	1.0	78.7
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	TGCAAATCCTTTACCCCCAG	50.0	50.0	58.2	57.8	944-2927 bp	1.0	78.7
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	ACTGCAAATCCTTTACCCCC	50.0	50.0	58.2	57.8	946-2927 bp	1.0	78.7
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	GCAAATCCTTTACCCCCAGT	50.0	50.0	58.2	57.8	943-2926 bp	1.0	78.7
petN-trnC-GCA_p5	GCCCAAGCGAGACTTACTAT	TGCAAATCCTTTACCCCCAG	50.0	50.0	57.1	57.8	943-2926 bp	1.0	72.8