Hotspot

trnK-UUU-rps16

LSC · 3938-4486 · 549 bp

Back to Eulalia

Pi 0.0146

MarkerSeek score 65.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 549
variable_sites: 8
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAAGAACA	50.0	47.6	58.3	58.2	681-688 bp	1.0	69.4
trnK-UUU-rps16_p2	CATAGCCGCACTTAAAAGCC	GGTGCTCAACCTACAAGAACA	50.0	47.6	57.5	58.2	686-693 bp	1.0	67.9
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAAGAAC	50.0	50.0	58.3	56.4	681-688 bp	1.0	65.6
trnK-UUU-rps16_p4	CGCACTTAAAAGCCGAGTAC	GGTGCTCAACCTACAAGAACA	50.0	47.6	57.2	58.2	680-687 bp	1.0	65.4
trnK-UUU-rps16_p5	TCTACCAAGAGTCTGGACGA	GGTGCTCAACCTACAAGAACA	50.0	47.6	57.1	58.2	737-744 bp	1.0	64.6