Hotspot

trnG-GCC-trnfM-CAU

LSC · 39855-40032 · 178 bp

Back to Eugenia

Pi 0.0549

MarkerSeek score 75.70

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 14
pi: 0.0549
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	CTTTCGGGTTGTGAGACACA	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	597-1083 bp	1.0	87.8
trnG-GCC-trnfM-CAU_p2	CTTTCGGGTTGTGAGACACA	CAGGGGCAACAAGATCAAGT	50.0	50.0	58.1	58.1	822-1309 bp	1.0	87.4
trnG-GCC-trnfM-CAU_p3	CTTTCGGGTTGTGAGACACA	TTCGGGAAATGGTTCATGCA	50.0	45.0	58.1	58.1	852-1339 bp	1.0	87.3
trnG-GCC-trnfM-CAU_p4	CTTTCGGGTTGTGAGACACA	ACGTATGCGGCTTTCTTGAT	50.0	45.0	58.1	58.0	1274-1761 bp	1.0	87.0
trnG-GCC-trnfM-CAU_p5	CTTTCGGGTTGTGAGACACA	ATTCACGTATGCGGCTTTCT	50.0	45.0	58.1	58.0	1278-1765 bp	1.0	87.0