Hotspot

psbZ-trnG-GCC

LSC · 39218-39783 · 566 bp

Back to Eugenia

Pi 0.0521

MarkerSeek score 77.50

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 33
pi: 0.0521
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	GACGGTTGGTCGAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.9	57.8	515-1001 bp	1.0	85.1
psbZ-trnG-GCC_p2	TTTGCTTCTCCTGACGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	527-1013 bp	1.0	82.9
psbZ-trnG-GCC_p3	ATTTGCTTCTCCTGACGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	528-1014 bp	1.0	81.6
psbZ-trnG-GCC_p4	ACGGTTGGTCGAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	514-1000 bp	1.0	81.4
psbZ-trnG-GCC_p5	GTATTTGCTTCTCCTGACGGT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	530-1016 bp	1.0	77.9