Hotspot

trnD-GUC-trnY-GUA

LSC · 32890-33329 · 440 bp

Back to Eugenia

Pi 0.0409

MarkerSeek score 74.00

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 440
variable_sites: 27
pi: 0.0409
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTTGATTCCGTCGGGACTG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.0	57.8	566-593 bp	1.0	82.0
trnD-GUC-trnY-GUA_p2	ATTTGATTCCGTCGGGACTG	GGCAATATGTCTACGCTGGT	50.0	50.0	58.0	57.8	565-592 bp	1.0	82.0
trnD-GUC-trnY-GUA_p3	ATTTGATTCCGTCGGGACTG	GGGGACGGACTGTAAATTCG	50.0	55.0	58.0	58.4	587-614 bp	1.0	81.0
trnD-GUC-trnY-GUA_p4	ATTTGATTCCGTCGGGACTG	GGGACGGACTGTAAATTCGT	50.0	50.0	58.0	57.6	586-613 bp	1.0	80.8
trnD-GUC-trnY-GUA_p5	ATTTGATTCCGTCGGGACTG	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.0	58.4	620-647 bp	1.0	80.3