Hotspot

atpH-atpI

LSC · 14913-16028 · 1116 bp

Back to Eugenia

Pi 0.0319

MarkerSeek score 69.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1116
variable_sites: 52
pi: 0.0319
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1163-1173 bp	1.0	83.5
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	1164-1174 bp	1.0	82.3
atpH-atpI_p3	TAACCCAGCAGCAATAACGG	CGAATCCATGGAGGGTCATC	50.0	55.0	58.3	57.8	1176-1186 bp	1.0	81.7
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1194-1204 bp	1.0	80.6
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1195-1205 bp	1.0	79.4