Hotspot

psbI-trnS-GCU

LSC · 8661-8814 · 154 bp

Back to Eugenia

Pi 0.0602

MarkerSeek score 76.70

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 154
variable_sites: 11
pi: 0.0602
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	TTTTTCGTACCGAGGGTTCG	50.0	50.0	57.1	58.2	166-214 bp	1.0	79.3
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	222-270 bp	1.0	79.2
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGA	GAGTTTTTCGTACCGAGGGT	50.0	50.0	57.1	57.6	169-217 bp	1.0	77.4
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGAA	TTTTTCGTACCGAGGGTTCG	47.6	50.0	57.8	58.2	166-214 bp	1.0	76.9
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	47.6	50.0	57.8	58.1	222-270 bp	1.0	76.8