Hotspot

ycf3-trnS-GGA

LSC · 44259-45193 · 935 bp

Back to Euchiton

Pi 0.00321
MarkerSeek score 56.50
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
935
variable_sites
3
pi
0.00321
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 1068-1069 bp 1.0 66.1
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 1069-1070 bp 1.0 66.1
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1106-1107 bp 1.0 65.9
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1107-1108 bp 1.0 65.9
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA AGTTCCAATGCTACGCCTTC 45.0 50.0 56.4 58.3 1070-1071 bp 1.0 65.5