Hotspot

trnT-GGU-psbD

LSC · 31208-32483 · 1276 bp

Back to Euchiton

Pi 0.00549

MarkerSeek score 62.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1276
variable_sites: 7
pi: 0.00549
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGACCCGTTCAGGAATTCA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.8	58.0	1475-1483 bp	1.0	79.0
trnT-GGU-psbD_p2	CAGTGACCCGTTCAGGAATT	GAAACGGTCCCTACGTAACC	50.0	55.0	57.8	58.0	1477-1485 bp	1.0	78.9
trnT-GGU-psbD_p3	CGTAAGCCCTCGGTTCAAAT	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1411-1419 bp	1.0	78.3
trnT-GGU-psbD_p4	TACAGTGACCCGTTCAGGAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1479-1487 bp	1.0	78.1
trnT-GGU-psbD_p5	TAAGCCCTCGGTTCAAATCC	GAAACGGTCCCTACGTAACC	50.0	55.0	57.6	58.0	1409-1417 bp	1.0	77.1