Hotspot

trnG-UCC-trnT-GGU

LSC · 30723-31144 · 422 bp

Back to Euchiton

Pi 0.00476

MarkerSeek score 51.00

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 422
variable_sites: 2
pi: 0.00476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	AATCAAACCGAAAGACCCCT	ATTTGAACCGAGGGCTTACG	45.0	50.0	57.0	58.3	549-552 bp	1.0	70.7
trnG-UCC-trnT-GGU_p2	ATCAAACCGAAAGACCCCTT	ATTTGAACCGAGGGCTTACG	45.0	50.0	57.0	58.3	548-551 bp	1.0	70.7
trnG-UCC-trnT-GGU_p3	ATCAAACCGAAAGACCCCTT	AGGGCTTACGCTTTACACTC	45.0	50.0	57.0	57.6	538-541 bp	1.0	69.8
trnG-UCC-trnT-GGU_p4	ATCAAACCGAAAGACCCCTT	GAGGGCTTACGCTTTACACT	45.0	50.0	57.0	57.6	539-542 bp	1.0	69.8
trnG-UCC-trnT-GGU_p5	ATCAAACCGAAAGACCCCTT	GGGCTTACGCTTTACACTCT	45.0	50.0	57.0	57.6	537-540 bp	1.0	69.8