Hotspot

rps16-trnQ-UUG

LSC · 6163-7163 · 1001 bp

Back to Euchiton

Pi 0.00406

MarkerSeek score 62.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1001
variable_sites: 4
pi: 0.00406
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.0	58.1	1089-1111 bp	1.0	80.8
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1074-1096 bp	1.0	79.8
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1088-1110 bp	1.0	77.9
rps16-trnQ-UUG_p4	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1138-1160 bp	1.0	76.8
rps16-trnQ-UUG_p5	GGGATCGTGTCCTTCAAGTC	TTCGGAGGTTCGAATCCTTC	55.0	50.0	58.0	57.3	1093-1115 bp	1.0	76.0