Hotspot

ycf1

SSC · 129237-134870 · 5634 bp

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Pi 0.0114

MarkerSeek score 58.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5634
variable_sites: 403
pi: 0.0114
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCAACCTGTCTCGATGTAA	TCCATTACTCTAGATGTATTCGA	45.0	34.8	55.9	53.9	184 bp	1.0	48.0
ycf1_p2	TCCAACCTGTCTCGATGTAA	TCCATTACTCTAGATGTATTCGAA	45.0	33.3	55.9	54.7	184 bp	1.0	48.0
ycf1_p3	ATCCAACCTGTCTCGATGTA	TCCATTACTCTAGATGTATTCGA	45.0	34.8	55.7	53.9	185 bp	1.0	48.0
ycf1_p4	TATCCAACCTGTCTCGATGT	TCCATTACTCTAGATGTATTCGA	45.0	34.8	55.7	53.9	186 bp	1.0	48.0
ycf1_p5	TCCAACCTGTCTCGATGTAA	ATCCATTACTCTAGATGTATTCGA	45.0	33.3	55.9	54.5	185 bp	1.0	48.0