Hotspot

rps19

LSC · 88626-88904 · 279 bp

Back to Eucalyptus

Pi 0.00606

MarkerSeek score 31.30

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 12
pi: 0.00606
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TGCTGCTGCAGAATAAACCA	CGTCGCCGTAGTAAATAGGG	45.0	55.0	58.1	58.0	601-625 bp	0.966667	86.0
rps19_p2	CTTCTTGCAGACCATCGGAA	CGTCGCCGTAGTAAATAGGG	50.0	55.0	57.9	58.0	973-1000 bp	0.966667	84.8
rps19_p3	TCTATGCGTGCAATTCCCTC	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.0	58.1	1067-1094 bp	0.966667	84.5
rps19_p4	TCTATGCGTGCAATTCCCTC	AAAAAGTTGGGTAGAGCCGG	50.0	50.0	58.0	58.1	1265-1292 bp	0.966667	83.5
rps19_p5	TCTATGCGTGCAATTCCCTC	CGTCGCCGTAGTAAATAGGG	50.0	55.0	58.0	58.0	1058-1085 bp	0.933333	83.3