Hotspot

ndhF

SSC · 115470-117707 · 2238 bp

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Pi 0.006

MarkerSeek score 49.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 100
pi: 0.006
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AAAGATATAAGAGCCGGTGA	50.0	40.0	58.0	53.5	2586-2927 bp	0.966667	52.1
ndhF_p2	ACAGGAACAAGAGGGATCCA	AAGATATAAGAGCCGGTGAA	50.0	40.0	58.0	53.5	2585-2926 bp	0.966667	52.1
ndhF_p3	AAGAGGGATCCACCGAAGAA	AAGATATAAGAGCCGGTGAA	50.0	40.0	58.0	53.5	2577-2918 bp	0.966667	52.1
ndhF_p4	GCAGAATACCGTCACCCATT	AAAGATATAAGAGCCGGTGA	50.0	40.0	58.0	53.5	2338-2889 bp	0.966667	51.3
ndhF_p5	GCAGAATACCGTCACCCATT	AAGATATAAGAGCCGGTGAA	50.0	40.0	58.0	53.5	2337-2888 bp	0.966667	51.3