Hotspot

rpl32-EARO_CP_t030

SSC · 118794-119392 · 599 bp

Back to Eucalyptus

Pi 0.0249

MarkerSeek score 81.40

Primer available yes

Label rpl32-EARO_CP_t030

Feature Metadata

label_name: rpl32-EARO_CP_t030
feature_type: igs
region: SSC
length_bp: 599
variable_sites: 75
pi: 0.0249
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-EARO_CP_t030_p1	GCGAAGTCTCTTTCTACCGG	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.1	58.0	664-692 bp	1.0	91.2
rpl32-EARO_CP_t030_p2	GCGAAGTCTCTTTCTACCGG	CAGCGTGTCTACCGATTTCA	55.0	50.0	58.1	58.0	665-693 bp	1.0	91.2
rpl32-EARO_CP_t030_p3	GCGAAGTCTCTTTCTACCGG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.1	57.9	674-702 bp	1.0	90.3
rpl32-EARO_CP_t030_p4	GTATATCGGGCAGCGTTGAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	697-725 bp	0.933333	88.2
rpl32-EARO_CP_t030_p5	GTATATCGGGCAGCGTTGAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	698-726 bp	0.933333	88.1