Hotspot

ndhF-rpl32

SSC · 117708-118634 · 927 bp

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Pi 0.0125

MarkerSeek score 67.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 927
variable_sites: 83
pi: 0.0125
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAAATTGTGCCGATTTCGCA	CCGGTAGAAAGAGACTTCGC	45.0	55.0	58.0	58.1	1755-1834 bp	1.0	85.9
ndhF-rpl32_p2	AAGGGGAAATTGTGCCGATT	CCGGTAGAAAGAGACTTCGC	45.0	55.0	58.1	58.1	1760-1839 bp	1.0	85.6
ndhF-rpl32_p3	AGGGGAAATTGTGCCGATTT	CCGGTAGAAAGAGACTTCGC	45.0	55.0	58.1	58.1	1759-1838 bp	1.0	85.6
ndhF-rpl32_p4	GAAATTGTGCCGATTTCGCA	TTCAACGCTGCCCGATATAC	45.0	50.0	58.0	58.1	1722-1801 bp	1.0	85.6
ndhF-rpl32_p5	GGGAAATTGTGCCGATTTCG	CCGGTAGAAAGAGACTTCGC	50.0	55.0	58.1	58.1	1757-1836 bp	1.0	85.4