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psbE-petL

LSC · 69140-70422 · 1283 bp

Back to Eucalyptus

Pi 0.00892

MarkerSeek score 66.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1283
variable_sites: 68
pi: 0.00892
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGTCGTATCTTGGTCAGACC	50.0	50.0	57.9	56.4	1448-1481 bp	1.0	73.0
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.9	56.3	1401-1434 bp	1.0	72.5
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGGTCAGACC	50.0	50.0	57.8	56.4	1371-1404 bp	1.0	72.5
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AGTCGTATCTTGGTCAGACC	45.0	50.0	58.2	56.4	1457-1490 bp	1.0	72.0
psbE-petL_p5	TGCTGACGAATAACCAACCC	AGTCGTATCTTGGTCAGACC	50.0	50.0	58.2	56.4	1462-1495 bp	1.0	71.9