Hotspot

rbcL-accD

LSC · 60458-61173 · 716 bp

Back to Eucalyptus

Pi 0.00894

MarkerSeek score 67.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 33
pi: 0.00894
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	ACTTAGCCCACACCTGTATTC	55.0	47.6	58.0	57.4	859-927 bp	0.966667	71.3
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TTTACTTAGCCCACACCTGT	55.0	45.0	58.0	56.4	862-930 bp	0.966667	71.2
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	ACTTAGCCCACACCTGTATTC	55.0	47.6	57.7	57.4	845-913 bp	1.0	70.8
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	TTTACTTAGCCCACACCTGT	55.0	45.0	57.7	56.4	848-916 bp	1.0	70.7
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	ACTTAGCCCACACCTGTATT	55.0	45.0	58.0	56.1	859-927 bp	0.966667	69.3