Hotspot

ndhC-EARO_CP_t019

LSC · 54634-55147 · 514 bp

Back to Eucalyptus

Pi 0.0152

MarkerSeek score 65.50

Primer available yes

Label ndhC-EARO_CP_t019

Feature Metadata

label_name: ndhC-EARO_CP_t019
feature_type: igs
region: LSC
length_bp: 514
variable_sites: 48
pi: 0.0152
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-EARO_CP_t019_p1	AATCGGGGCTAAAATTCCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	620-680 bp	1.0	85.9
ndhC-EARO_CP_t019_p2	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	628-688 bp	1.0	85.8
ndhC-EARO_CP_t019_p3	CGGGGCTAAAATTCCGGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.5	57.8	617-677 bp	1.0	84.2
ndhC-EARO_CP_t019_p4	AATCGGGGCTAAAATTCCGG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.3	57.5	619-679 bp	1.0	83.2
ndhC-EARO_CP_t019_p5	CCTTTGCTAATCGGGGCTAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.7	57.5	627-687 bp	1.0	83.0