Pi
0.00982
MarkerSeek score
73.60
Primer available
yes
Label
EARO_CP_t016-EARO_CP_t017
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| EARO_CP_t016-EARO_CP_t017_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1235-1352 bp | 1.0 | 87.5 |
| EARO_CP_t016-EARO_CP_t017_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1243-1360 bp | 1.0 | 86.9 |
| EARO_CP_t016-EARO_CP_t017_p3 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1267-1384 bp | 1.0 | 86.5 |
| EARO_CP_t016-EARO_CP_t017_p4 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 1275-1392 bp | 1.0 | 86.0 |
| EARO_CP_t016-EARO_CP_t017_p5 | GCGATGCTCTAACCTCTGAG | AGTCCGTAGCGTCTACCAAT | 55.0 | 50.0 | 57.9 | 58.2 | 1249-1366 bp | 1.0 | 85.6 |