Hotspot

psaA-ycf3

LSC · 45268-46054 · 787 bp

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Pi 0.0135

MarkerSeek score 71.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 48
pi: 0.0135
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	913-999 bp	1.0	86.9
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	894-980 bp	1.0	86.8
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	AGCGCAGAATTGGTTGAAGA	50.0	45.0	58.1	58.1	855-941 bp	1.0	86.7
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	836-922 bp	1.0	86.6
psaA-ycf3_p5	TGAGAAATGACCCGGTTTGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	915-1001 bp	1.0	86.2