Hotspot

psbZ-EARO_CP_t013

LSC · 39207-39877 · 671 bp

Back to Eucalyptus

Pi 0.0186

MarkerSeek score 77.20

Primer available yes

Label psbZ-EARO_CP_t013

Feature Metadata

label_name: psbZ-EARO_CP_t013
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 72
pi: 0.0186
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-EARO_CP_t013_p1	GACGGTTGGTCGAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.9	57.8	548-1020 bp	1.0	88.3
psbZ-EARO_CP_t013_p2	ACGGTTGGTCGAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	547-1019 bp	1.0	84.6
psbZ-EARO_CP_t013_p3	TTTGCTTCTCCTGACGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	560-1012 bp	0.966667	84.0
psbZ-EARO_CP_t013_p4	ATTTGCTTCTCCTGACGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	561-1013 bp	0.966667	82.7
psbZ-EARO_CP_t013_p5	GACGGTTGGTCGAGTAACAA	ACTTTAACCTGGGCGGATAG	50.0	50.0	57.9	57.0	583-1055 bp	0.966667	79.7