Hotspot

EARO_CP_t010-EARO_CP_t011

LSC · 33510-34419 · 910 bp

Back to Eucalyptus

Pi 0.00862

MarkerSeek score 67.30

Primer available yes

Label EARO_CP_t010-EARO_CP_t011

Feature Metadata

label_name: EARO_CP_t010-EARO_CP_t011
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 61
pi: 0.00862
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
EARO_CP_t010-EARO_CP_t011_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	1019-1061 bp	1.0	87.4
EARO_CP_t010-EARO_CP_t011_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	998-1040 bp	1.0	87.1
EARO_CP_t010-EARO_CP_t011_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	995-1037 bp	1.0	86.9
EARO_CP_t010-EARO_CP_t011_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	981-1023 bp	1.0	86.8
EARO_CP_t010-EARO_CP_t011_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	960-1002 bp	1.0	86.7