Hotspot

atpH-atpI

LSC · 14907-16017 · 1111 bp

Back to Eucalyptus

Pi 0.00715

MarkerSeek score 74.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1111
variable_sites: 64
pi: 0.00715
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1167-1230 bp	1.0	83.9
atpH-atpI_p2	AATAGAAGCAAGCCCGACAG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.0	58.5	1201-1264 bp	1.0	83.8
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TAGGTGAATCCATGGAGGGT	50.0	50.0	58.0	57.4	1174-1237 bp	1.0	83.4
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	1140-1203 bp	1.0	83.4
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1223-1286 bp	1.0	83.2