Hotspot

EARO_CP_t004-EARO_CP_t005

LSC · 8965-9751 · 787 bp

Back to Eucalyptus

Pi 0.00987

MarkerSeek score 67.30

Primer available yes

Label EARO_CP_t004-EARO_CP_t005

Feature Metadata

label_name: EARO_CP_t004-EARO_CP_t005
feature_type: igs
region: LSC
length_bp: 787
variable_sites: 51
pi: 0.00987
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
EARO_CP_t004-EARO_CP_t005_p1	CAATCCGACGCTTTAGTCCA	AAATCAAACCGAGGGATCCC	50.0	50.0	58.0	57.6	893-930 bp	1.0	83.5
EARO_CP_t004-EARO_CP_t005_p2	AATCCGACGCTTTAGTCCAC	AAATCAAACCGAGGGATCCC	50.0	50.0	58.0	57.6	892-929 bp	1.0	83.5
EARO_CP_t004-EARO_CP_t005_p3	ACGGAAAGAGAGGGATTCGA	AAATCAAACCGAGGGATCCC	50.0	50.0	58.2	57.6	947-984 bp	1.0	82.0
EARO_CP_t004-EARO_CP_t005_p4	AGCAATCCGACGCTTTAGTC	AAATCAAACCGAGGGATCCC	50.0	50.0	58.4	57.6	895-932 bp	1.0	80.7
EARO_CP_t004-EARO_CP_t005_p5	CGACGCTTTAGTCCACTCAG	AAATCAAACCGAGGGATCCC	55.0	50.0	58.4	57.6	888-925 bp	1.0	80.6