Hotspot

petB

LSC · 75086-76505 · 1420 bp

Back to Eschenbachia

Pi 0.0113

MarkerSeek score 52.60

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1420
variable_sites: 16
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TGGCAATGGCTTTATTTGCG	GCACATATCTGCACAGACCA	45.0	50.0	58.0	58.0	1869-1885 bp	1.0	81.0
petB_p2	GCAATGGCTTTATTTGCGGT	GCACATATCTGCACAGACCA	45.0	50.0	58.0	58.0	1867-1883 bp	1.0	81.0
petB_p3	TGGCAATGGCTTTATTTGCG	AAGGGCACATATCTGCACAG	45.0	50.0	58.0	58.0	1873-1889 bp	1.0	80.9
petB_p4	GCAATGGCTTTATTTGCGGT	AAGGGCACATATCTGCACAG	45.0	50.0	58.0	58.0	1871-1887 bp	1.0	80.8
petB_p5	TGGCAATGGCTTTATTTGCG	ATTGCTTTCTGGGTCGTCTC	45.0	50.0	58.0	57.9	2243-2264 bp	1.0	80.3