Hotspot

ndhE-ndhG

SSC · 117397-117625 · 229 bp

Back to Eschenbachia

Pi 0.0219

MarkerSeek score 65.50

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 229
variable_sites: 5
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	CACATAAGCGCCCTAACCAT	TAATCGGCGCAATTGCTGTA	50.0	45.0	58.0	58.3	358-359 bp	1.0	78.3
ndhE-ndhG_p2	AGCGCCCTAACCATATTTCG	TAATCGGCGCAATTGCTGTA	50.0	45.0	58.1	58.3	352-353 bp	1.0	77.7
ndhE-ndhG_p3	GCGCCCTAACCATATTTCGA	TAATCGGCGCAATTGCTGTA	50.0	45.0	58.1	58.3	351-352 bp	1.0	77.6
ndhE-ndhG_p4	CAAGACACATAAGCGCCCTA	TAATCGGCGCAATTGCTGTA	50.0	45.0	57.7	58.3	363-364 bp	1.0	76.1
ndhE-ndhG_p5	AGACACATAAGCGCCCTAAC	TAATCGGCGCAATTGCTGTA	50.0	45.0	57.7	58.3	361-362 bp	1.0	76.1