Hotspot

ccsA-ndhD

SSC · 114679-114947 · 269 bp

Back to Eschenbachia

Pi 0.0335
MarkerSeek score 61.00
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
269
variable_sites
9
pi
0.0335
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGGCTACATAGTTATGGTTCA TCGCTATCAGTTGACAAGGT 40.9 45.0 56.6 56.6 366-370 bp 1.0 43.4
ccsA-ndhD_p2 TGGGCTACATAGTTATGGTTCA TCAGTTGACAAGGTTGAAGGT 40.9 42.9 56.6 57.4 360-364 bp 1.0 42.0
ccsA-ndhD_p3 TGGGCTACATAGTTATGGTTCA TCGCTATCAGTTGACAAGGTT 40.9 42.9 56.6 57.3 366-370 bp 1.0 42.0
ccsA-ndhD_p4 TGGGCTACATAGTTATGGTTCA TTCGCTATCAGTTGACAAGGT 40.9 42.9 56.6 57.3 367-371 bp 1.0 42.0
ccsA-ndhD_p5 TTGGGCTACATAGTTATGGTTCA TCGCTATCAGTTGACAAGGT 39.1 45.0 57.3 56.6 367-371 bp 1.0 42.0