Hotspot

rpl32-trnL-UAG

SSC · 112682-113513 · 832 bp

Back to Eschenbachia

Pi 0.021

MarkerSeek score 68.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 832
variable_sites: 17
pi: 0.021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAAAGGATATTGGGCGTCGT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	951-961 bp	1.0	82.7
rpl32-trnL-UAG_p2	GAAAGGATATTGGGCGTCGT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	952-962 bp	1.0	82.7
rpl32-trnL-UAG_p3	GAAAGGATATTGGGCGTCGT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	961-971 bp	1.0	81.7
rpl32-trnL-UAG_p4	GAAAGGATATTGGGCGTCGT	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.1	57.8	960-970 bp	1.0	81.2
rpl32-trnL-UAG_p5	GAAAGGATATTGGGCGTCGT	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.1	57.8	959-969 bp	1.0	81.2